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核酸標記探針

產品介紹

GeneCopoeia 最新推出一係列熒光素和生物素標記dUTP和UTP探針。這些探針可廣泛應用於FISH, TUNEL, DNA microarray等應用。我們的探針采用熒光亮度強,光穩定性好的Andy Fluor染料標記,並且在染料和dUTP及UTP之間設計一條特殊的X-linker以確保在酶的作用下更有效的插入DNA或RNA。

產品特點

◆熒光信號強,穩定性好,背景低;
◆多種顏色可供選擇,適合多重染色;
◆適用性廣:適用於各種酶的標記。​

 

Figure 1. Fluorescence in situ Hybridization (FISH) of CEP7/MET probes labeled with Andy Fluor dyes. Human Chromosome 7 Enumeration (CEP7) and Human c-Met gene (MET) probes were labeled with Andy Fluor 488-X-dUTP (green) and Andy Fluor 568-X-dUTP (magenta), respectively, using nick translation. The labeled probes were mixed together in hybridization buffers with blocking DNA, and incubated with HT1080 cells on slides at 37°C overnight. The slides was washed and mounted with AntiFade solution containing DAPI (blue), then visualized with an Epi-Fluorescence microscope.

TUNEL-biotin-11-UTP mouse tongue

Figure 2. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue using Biotin-11-dUTP. Left: Cells were labeled with Biotin-11-dUTP, followed by detection with AndyFluor™ 488-streptavidin (green). Nuclei were also counterstained with DAPI (blue). Right: Cells were labeled with Biotin-11-dUTP, followed by detection with horse radish peroxidase (HRP).

訂購

核酸標記探針

購買 貨號 產品名稱 激發/發射波長(nm) 規格 價格(¥)
C401B 5-Aminoallyl-dUTP, lyophilized powder 1 µmol 1200
C402B 5-Aminoallyl-UTP, lyophilized powder 1 µmol 1100
C403B Biotin-11-dUTP, lyophilized powder 50 nmol 1800
C404B Biotin-11-UTP, lyophilized powder 50 nmol 1800
C405B 5-FAM-dUTP, lyophilized powder 495/518 25 nmol 1400
C406B 5-FAM-X-dUTP, lyophilized powder 495/518 25 nmol 1800
C407B 5-FAM-UTP, lyophilized powder 495/518 25 nmol 1400
C408B 5-FAM-X-UTP, lyophilized powder 495/518 25 nmol 1800
C409B Andy Fluor 488-X-dUTP, lyophilized powder 500/525 25 nmol 2800
C410B Andy Fluor 488-X-UTP, lyophilized powder 500/525 25 nmol 2800
C411B Andy Fluor 555-X-dUTP, lyophilized powder 553/565 25 nmol 2800
C412B Andy Fluor 555-X-UTP, lyophilized powder 553/565 25 nmol 2800
C413B Andy Fluor 568-X-dUTP, lyophilized powder 578/602 25 nmol 2800
C414B Andy Fluor 568-X-UTP, lyophilized powder 578/602 25 nmol 2800
C415B Andy Fluor 594-X-dUTP, lyophilized powder 590/615 25 nmol 2800
C416B Andy Fluor 594-X-UTP, lyophilized powder 590/615 25 nmol 2800
C417B Andy Fluor 647-X-dUTP, lyophilized powder 650/665 25 nmol 2800
C418B Andy Fluor 647-X-UTP, lyophilized powder 650/665 25 nmol 2800
C419B Cy3-X-dUTP, lyophilized powder 553/565 25 nmol 2400
C420B Cy3-X-UTP, lyophilized powder 553/565 25 nmol 2400
C421B Cy5-X-dUTP, lyophilized powder 650/665 25 nmol 2400
C422B Cy5-X-UTP, lyophilized powder 650/665 25 nmol 2400